2024-07-252024-06-252024-07-252019-12-05https://repositorio.ifal.edu.br/handle/123456789/561In the present work we describe the development of an electrode that uses xanturenic acid (AX) for the first time as an electrochemical probe, through a simple and efficient Aano-activated, nanomaterial modified carbon printed electrode immobilized with aptamers for the detection of aflotoxin. B1 (AFB1). The sensor surface was characterized by voltammetric techniques such as differential pulse voltammetry (VPD) and cyclic voltammetry (VC), which were used to study the behavior of modified electrodes, to optimize experimental parameters and obtain kinetic parameters. as a study of speed and pH. The electroactive species, quinone / hydroquinone, was electrogenerated in situ on the modified nanomaterials and AX electrode after cycles in the potential range between 0.5 and -1.0 vs. Ag at first sweep in 0.1 mol L-1 phosphate buffer (pH 5.5) with sweep rate of 50 mV s-1. After the activation process, the potential range around the redox quinone / hydroquinone pair was selected and the voltammograms were recorded at different speeds (5-20 mV s-1), then aptamer immobilization was performed, applied with the intention of to test the electrocatalytic activity for AFB1 oxidation. In addition, the electroactive species generated on the electrode surface showed inhibition against the immobilized aptamer when reacting with AFB1, causing a charge retention. Thus, from the results obtained the electrochemical aptensor developed showed a promising alternative to be applied in tests with real samples.Acesso AbertoQuímicaSensor eletroquímicoÁcido XanturênicoAflotoxina B1Xanturenic AcidElectrochemical sensorAflotoxin B1Trabalho de Conclusão de CursoCNPQ::CIENCIAS EXATAS E DA TERRA::QUIMICA