TCC Horticultura

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Navegando TCC Horticultura por Orientador "Barbosa, Tiago Jorge de Araújo"

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    Potencial antifúngico de extratos vegetais no controle in vitro de Lasiodiplodia spp.
    (INSTITUTO FEDERAL DE ALAGOAS - Ifal, 2026-02-04) Silva, José Fabio da; Barbosa, Tiago Jorge de Araújo; http://lattes.cnpq.br/4598181798521668; Carnaúba, Juliana Paiva; https://orcid.org/0000-0001-7246-8639; http://lattes.cnpq.br/9758718992783544; Santos, Maria Fabíola Moraes da Assumpção; http://lattes.cnpq.br/7941533987806732
    This study aimed to evaluate the effect of plant extracts from four medicinal species boldo-falso (Plectranthus barbatus), falsa-mirra (Tetradenia riparia), melão-de-são-caetano (Momordica charantia), and mastruz (Dysphania ambrosioides) on the in vitro mycelial growth of the phytopathogenic fungi Lasiodiplodia theobromae, L. pseudotheobromae, and L. brasiliensis. The experiments were conducted at the Plant Health Laboratory of the Federal Institute of Alagoas - Maragogi Campus, using fungal isolates obtained from comercial coconut plantations in the state of Alagoas, exhibiting symptoms of leaf blight. The extracts were prepared from leaves of adult plants of the medicinal species, which, after collection, were dried, macerated, subjected to alcoholic extraction, then filtered, and the ethanol evaporated in a water bath. The extracts produced were diluted to concentrations of 5, 10, and 15%, added to PDA medium in Petri dishes (10x90 mm), where the fungi were inoculated. Mycelial growth was measured daily using calipers until one of the treatments reached the edge of the plates. The experimental design adopted was completely randomized, conducting three distinct experiments, each targeting a different fungus. The data were subjected to analysis of variance, and the means were compared using Tukey's test (P<0.05) with the aid of the SISVAR software. The plant extracts of boldo-falso, falsa-mirra, melão-de-são-caetano, and mastruz have an antifungal effect on the in vitro mycelial growth of the fungi L. theobromae, L. pseudotheobromae and L. brasiliensis. At concentrations of 10% or higher, the four plant extracts promoted the complete inhibition of mycelial growth of the three Lasiodiplodia spp. species, regardless of the evaluation period, suggesting the presence of bioactive compounds with fungistatic and fungicidal action.